Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: TAC caused similar left ventricular hypertrophy, fibrosis and accumulation of immune cells in WT and CD40 KO mice. Post-TAC survival analysis of WT (n = 41) and CD40 KO (n = 37) mice (A). The ratios of LV and LA weight to tibial length of WT and CD40 KO mice under control or TAC condition (n = 9-13), Sham indicates no actual TAC (B, C). Representative images and summary data for LV myocyte cross-sectional area determined by FITC-conjugated wheat germ agglutinin (WGA) staining (n = 5-7) (D). Representative images and quantitative data of LV fibrosis by Sirius red/Fast green staining (n = 4-7) (E). CD45 immunostaining (red) and quantitative data of LV leukocyte infiltration (n = 5-6) (F). Survival rate was analyzed by Kaplan-Meier method and compared by log-rank test. All quantitative data are reported as means ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Control, Staining, Immunostaining

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: TAC caused comparable LV dysfunction in WT and CD40 KO mice. Representative echocardiograms of each group (A). Summary data for LV ejection fraction (EF%), fractional shortening (FS%), LV end-diastolic and end-systolic diameter (LV-EDD, LV-ESD) and heart rate (n = 9-13) in each group (B–F). LV pressure of WT (n = 5) and CD40 KO (n = 5) mice were measured at 8 weeks after TAC, representative invasive pressure curve(G) with quantifications of end-systolic pressure (ESP)(I) and end-diastolic pressure (EDP)(J). Representative dp/dt curve(H) with quantifications of dp/dtmax (maximal rate of change in systolic pressure over time) (L) and dp/dtmin (minimal rate of change in pressure over time) (M). The rate-pressure product (K) was calculated by multiplying the heart rate by the LV end-systolic pressure. Western blot of β-MHC and vinculin (loading control, n = 3-4) (N, O). All quantitative data are reported as mean ± SEM. The statistical significance was assessed using two-tailed Student's unpaired t tests(I-M). Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis (B–F, N, O). ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Western Blot, Control, Two Tailed Test

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO aggravated TAC-induced increase of lung weight. The ratios of lung wet weight, lung dry weight, lung water weight to tibial length (A-C). Lung water content percentage ((lung wet weight – lung dry weight)/lung wet weight × 100%) of each group mice (n = 7-13) (D). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques:

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO caused robust pulmonary microvascular thrombosis in mice after TAC. Representative images of hematoxylin and eosin (HE) staining of lung tissues from the experimental groups(A) . Representative immunostaining images of Carstairs staining of the lung (Fibrin: bright red, platelets: gray blue or navy, collagen: bright blue), CD42c, CD61 and vWF in the lung (B-E). Quantitative data of CD42c immunofluorescence staining in the lung (n = 5-7) (F). The platelet counts in each group of mice (n = 8-14) (G). Mean platelet volume (n = 8-14) (H). Quantitative data of vWF in the lung (n = 5-6) (I). The CD42c and vWF staining were expressed as a percentage of positive staining area to the total area. All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Staining, Immunostaining, Immunofluorescence

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO exacerbated platelet aggregation and blood clot retraction in mice after TAC. The aggregation levels of WT and CD40 KO mouse platelets in response to 1.2 μg/ml collagen stimulation (n = 3-5) (A). The aggregation levels of WT and CD40 KO mouse platelets in response to 0.067U/ml thrombin stimulation (n = 5-6) (B). The aggregation levels of WT and CD40 KO mouse platelets in response to 3.4U/ml ADP stimulation (n = 4-5) (C). Clot retraction of WT and CD40 KO mouse platelets (n = 5-7) (D). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques:

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO aggravated TAC-induced pulmonary leukocyte infiltration. Representative images and quantitative data of CD45 + cells, Mac2 + cells and CD3 + cells in lungs (n = 5-7) (A-C). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques:

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO exacerbated pulmonary fibrosis and vessel remodeling in mice after TAC. Representative images (A-C) and quantitative data (D-F) of Masson's trichrome staining and smooth muscle α-actin (red) of the lung tissues. Quantitative RT-PCR result of pulmonary TGFβ (normalized to 18S) (G). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Staining, Quantitative RT-PCR

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: CD40 KO exacerbated pulmonary oxidative stress in mice with existing LV dysfunction. Representative images and Quantitative data of pulmonary dihydroethidium (DHE) staining in mice (n = 5) (A, D), IHC analysis of 3′-nitrotyrosine (B, E) and 4-hydroxynonenal (n = 4-6) (C, F). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Staining

Journal: Redox Biology

Article Title: Systolic pressure overload caused pulmonary oxidative stress, vessel remodeling and severe microvascular thrombosis in CD40 knockout mice through promoting platelet aggregation

doi: 10.1016/j.redox.2026.104122

Figure Lengend Snippet: TAC resulted in significant RV inflammation, fibrosis and cardiomyocyte hypertrophy in CD40 KO mice. The ratio of RV weight to tibial length of WT and CD40 KO mice under control or TAC condition (n = 9-13) (A). Western blot of β-MHC and loading control of vinculin (n = 3-4) (B, C)). Representative images and summary data for RV myocyte cross-sectional area determined by FITC-conjugated WGA staining (n = 6) (D). Representative images and quantitative data of RV fibrosis by Sirius red/Fast green staining (n = 5-6) (E). CD45 immunostaining (red) and quantitative data of RV leukocyte infiltration (n = 5-7) (F). All quantitative data are reported as mean ± SEM. Data were analyzed using one-way ANOVA followed by Bonferroni post hoc analysis. ns indicates nonsignificant ( p > 0.05),∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001,∗∗∗∗ p < 0.0001.

Article Snippet: Animals and experimental design: CD40 KO mice (B6.129P2– Cd40 tm1Kik /J; stock NO: 002928) and wild type (WT) C57BL/6J mice were purchased from Jackson Laboratory and Shanghai SLAC Laboratory Animal Co, Ltd. Based on the information provided by Jackson Laboratory, CD40 KO strain has been backcrossed to C57BL/6J mice for at least 10 generations.

Techniques: Control, Western Blot, Staining, Immunostaining

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: MDSC were isolated from the spleens of WT C57BL/6 mice without tumor inoculation (tumor-free; n = 5) or from the spleens and tumors of mice with tumors (diameter = 1 cm; n = 5) grown from subcutaneously injected MFC, RM-1 or LLC cells. CD40 + % MDSC were analyzed using flow cytometry after staining isolated MDSC with PE-conjugated anti-CD40 antibody (CD40 − PE; blue line) or PE-conjugated isotype-matched IgG control antibody (red line). a. Representative flow cytometry images showing minimal CD40 + cell detection in tumor-free mouse spleen, and a dramatic increase in CD40 + % MDSC after tumor formation from all three different types of cancer cells. The highest CD40 + % levels were detected in tumor tissues. b. CD40 + % quantification in different groups of mice. * p < 0.05 and ** p < 0.01, compared to the corresponding tumor tissues.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Isolation, Injection, Flow Cytometry, Staining, Control

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: Single cells dissociated from MFC tumors were stained with fluorophore-conjugated anti-CD11b, anti-Gr-1 and anti-CD40 antibodies. The gating strategy for FACS as well as the pre- and post-sorting percentages of CD40 high and CD40 low MDSC are shown.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Staining

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: Gene expression profiles of CD40 high MDSC and CD40 low MDSC were examined by microarray analysis. a. Scatter plot of microarray data showing genes that were more than two-fold upregulated (red), less than two fold changed (black) or downregulated by more than two fold (green) in CD40 high compared with CD40 low MDSC. b. Heat map representation of microarray data on genes of interest. Expression levels are indicated on a color scale where red represents higher expression and green lower expression. c. qRT-PCR analysis of eight genes comparing CD40 high and CD40 low MDSC expression levels. The expression level of each target gene relative to an internal control (β-actin) was determined using the 2 −ΔΔCT method. The fold change between the two cell groups is presented.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Gene Expression, Microarray, Expressing, Quantitative RT-PCR, Control

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: Flow cytometry analysis of the percentage of CD11b + Gr-1 + MDSC (MDSC%) within tumor tissues from WT ( n = 6) or KO ( n = 6) mice a and b. , and the CD40 + versus CD40 − MDSC subsets within WT tumor tissues ( c and d. n = 9). a) Representative flow cytometry images demonstrating the gating strategy and a higher MDSC% in tumor tissues from WT mice when compared with KO mice. b) Quantification of the MDSC% determined in a. c) Representative flow cytometry images demonstrating the gating strategy and a significantly higher percentage of CD40 + than CD40 − MDSC in WT tumor tissue. d) Quantification of the flow cytometry data shown in c.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Flow Cytometry

Journal: Oncotarget

Article Title: CD40 controls CXCR5-induced recruitment of myeloid-derived suppressor cells to gastric cancer

doi:

Figure Lengend Snippet: a. MFC were injected into WT ( n = 6) and KO ( n = 6) mice. Tumor growth was measured and is presented as tumor size at the indicated post-MFC inoculation time points. * p < 0.05, compared with tumors from CD40 KO mice. b. Fifteen days after MFC inoculation, the mice were sacrificed and tumors were excised and photographed. Tumors from WT mice were larger than tumors from KO mice. WT tumors were also irregularly shaped, with rough surfaces and ulcers.

Article Snippet: Syngenic age-matched CD40 knockout mice (KO) were purchased from the Jackson Laboratory (Maine, USA).

Techniques: Injection